Objective: To construct the expression plasmid AP33-pVE5523.Methods: The total RNA extracted from the trophozoites of T.vaginalis was used as template for the amplification of AP33 gene by using RT-PCR.The amplified products were ligated with expression plasmid pVE5523 of lactic acid bacteria(LAB).Then the recombination product AP33-pVE5523 was preserved in TOP10 bacteria.Results:The recombination product AP33-pVE5523 checked by PCR was correct.The DNA sequence analysis revealed that the homology with the gene of T.vaginalis adhesion protein AP33-1 was 100%.Conclusion: The expression plasmid AP33-pVE5523 was constructed successfully,which provides basis for the reaserch of AP33 expression in LAB.