ObjectiveTo investigate the cleaning quality of  frequently touched object surfaces in a hospital, so as to formulate effective evidencebased intervention measures.MethodsFrequently touched object surfaces in common wards, operating rooms, and intensive care unit(ICU) of the hospital between October 2014 and February 2015 were labeled by fluorescent adhesive agents 1-2 times a week, objects were labeled before patients’ discharge and at the end of operation, cleaning quality of frequently touched object surfaces were checked after routine cleaning.ResultsA total of 2 131 object surfaces were monitored, fluorescent adhesive agents on 1 732 objects were cleared, clearance rate was 81.3%，hygienic clean was qualified. 642,650,and 839 objects surfaces in common wards, operating rooms, and ICU were monitored respectively, the clearance rates were 82.1%,75.8%,and 84.9% respectively,  the clearance rates  among three wards were significantly different (χ2 =19.964，P＜0.001）. In common wards, fluorescent clearance rates of call buttons, power switches, and equipments were 51.3%,55.2%, and 58.5% respectively; in operating room,  fluorescent clearance rates of room wall, control panel of anesthesia machine, bedadjusting device, keyboards, and infusion pumps were 38.6%,48.8%,60.0%,61.3%,and 68.4%，respectively; in ICU, fluorescent clearance rates of equipments was 65.9%, all were unqualified.ConclusionThe environmental hygiene quality is generally qualified in this hospital, but the cleaning quality of frequently touched object surfaces needs to be further improved.